| 1 |  | Marlen Reusser | Movistar Team | 17:22m |
| 2 |  | Lotte Kopecky | Team SD Worx - Protime | +12s |
| 3 |  | Elisa Longo Borghini | UAE Team ADQ | +16s |
| 4 |  | Anna van der Breggen | Team SD Worx - Protime | +20s |
| 5 | | Lieke Nooijen | Team Visma | Lease a Bike | +24s |
| 6 |  | Anna Henderson | Lidl - Trek | +27s |
| 7 |  | Christina Schweinberger | Fenix-Deceuninck | +30s |
| 8 | | Alessia Vigilia | FDJ - SUEZ | +40s |
| 9 | | Monica Trinca Colonel | Liv AlUla Jayco | +41s |
| 10 | | Shirin van Anrooij | Lidl - Trek | +41s |
| 11 |  | Georgia Baker | Liv AlUla Jayco | +43s |
| 12 | | Elena Hartmann | Ceratizit-WNT Pro Cycling | +44s |
| 13 |  | Katrine Aalerud | Uno-X Mobility | +44s |
| 14 |  | Antonia Niedermaier | CANYON//SRAM zondacrypto | +48s |
| 15 |  | Sarah van Dam | Ceratizit-WNT Pro Cycling | +50s |
| 16 |  | Juliette Labous | FDJ - SUEZ | +51s |
| 17 |  | Ruth Edwards | Human Powered Health | +51s |
| 18 | | Lauretta Hanson | Lidl - Trek | +52s |
| 19 | | Franziska Brauße | Ceratizit-WNT Pro Cycling | +52s |
| 20 | | Pfeiffer Georgi | Team Picnic PostNL | +55s |
| 21 | | Lorena Wiebes | Team SD Worx - Protime | +55s |
| 22 |  | Alena Amialiusik | UAE Team ADQ | +57s |
| 23 |  | Rebecca Koerner | Uno-X Mobility | +57s |
| 24 | | Anouska Koster | Uno-X Mobility | +58s |
| 25 | | Josie Talbot | Liv AlUla Jayco | +58s |
| 26 | | Marianne Vos | Team Visma | Lease a Bike | +58s |
| 27 |  | Mireia Benito Pellicer | AG Insurance - Soudal Team | +59s |
| 28 | | Mareille Meijering | Movistar Team | +1:00m |
| 29 | | Aude Biannic | Movistar Team | +1:00m |
| 30 | | Silke Smulders | Liv AlUla Jayco | +1:02m |
| 31 | | Silvia Persico | UAE Team ADQ | +1:02m |
| 32 | | Sarah Gigante | AG Insurance - Soudal Team | +1:03m |
| 33 | | Justyna Czapla | CANYON//SRAM zondacrypto | +1:04m |
| 34 | | Eva van Agt | Team Visma | Lease a Bike | +1:05m |
| 35 | | Loes Adegeest | FDJ - SUEZ | +1:06m |
| 36 | | Megan Jastrab | Team Picnic PostNL | +1:07m |
| 37 | | Yara Kastelijn | Fenix-Deceuninck | +1:10m |
| 38 | | Amanda Spratt | Lidl - Trek | +1:10m |
| 39 | | Quinty Ton | Liv AlUla Jayco | +1:13m |
| 40 | | Soraya Paladin | CANYON//SRAM zondacrypto | +1:13m |
| 41 | | Lucinda Brand | Lidl - Trek | +1:14m |
| 42 |  | Marta Jaskulska | Ceratizit-WNT Pro Cycling | +1:14m |
| 43 |  | Urška Žigart | AG Insurance - Soudal Team | +1:15m |
| 44 | | Isabella Holmgren | Lidl - Trek | +1:15m |
| 45 |  | Viktoria Chladonova | Team Visma | Lease a Bike | +1:15m |
| 46 | | Kamilla Aasebo | Uno-X Mobility | +1:17m |
| 47 | | Pauliena Rooijakkers | Fenix-Deceuninck | +1:17m |
| 48 | | Eleonora Ciabocco | Team Picnic PostNL | +1:17m |
| 49 | | Marta Cavalli | Team Picnic PostNL | +1:17m |
| 50 | | Liane Lippert | Movistar Team | +1:18m |
| 51 | | Josie Nelson | Team Picnic PostNL | +1:19m |
| 52 | | Chiara Consonni | CANYON//SRAM zondacrypto | +1:20m |
| 53 |  | Kim Cadzow | EF Education-Oatly | +1:20m |
| 54 | | Lore De Schepper | AG Insurance - Soudal Team | +1:21m |
| 55 |  | Anniina Ahtosalo | Uno-X Mobility | +1:22m |
| 56 | | Fleur Moors | Lidl - Trek | +1:22m |
| 57 | | Alexandra Manly | AG Insurance - Soudal Team | +1:23m |
| 58 | | Sarah Roy | EF Education-Oatly | +1:24m |
| 59 | | Elena Cecchini | Team SD Worx - Protime | +1:25m |
| 60 |  | Ashleigh Moolman-Pasio | AG Insurance - Soudal Team | +1:26m |
| 61 | | Léa Curinier | FDJ - SUEZ | +1:27m |
| 62 | | Sara Casasola | Fenix-Deceuninck | +1:28m |
| 63 | | Gaia Masetti | AG Insurance - Soudal Team | +1:30m |
| 64 |  | Yuliia Biriukova | Laboral Kutxa - Fundación Euskadi | +1:30m |
| 65 | | Sara Martín | Movistar Team | +1:30m |
| 66 | | Dilyxine Miermont | Ceratizit-WNT Pro Cycling | +1:31m |
| 67 | | Eglantine Rayer | FDJ - SUEZ | +1:31m |
| 68 | | Tamara Dronova-Balabolina | Roland | +1:31m |
| 69 | | Evita Muzic | FDJ - SUEZ | +1:31m |
| 70 | | Francesca Barale | Team Picnic PostNL | +1:31m |
| 71 | | Alice Towers | CANYON//SRAM zondacrypto | +1:32m |
| 72 | | Silvia Zanardi | Human Powered Health | +1:32m |
| 73 | | Carina Schrempf | Fenix-Deceuninck | +1:33m |
| 74 | | Aniek van Alphen | Fenix-Deceuninck | +1:33m |
| 75 |  | Ana Vitoria Magalhaes | Movistar Team | +1:33m |
| 76 | | Mikayla Harvey | Team SD Worx - Protime | +1:33m |
| 77 | | Maud Oudeman | Team Visma | Lease a Bike | +1:33m |
| 78 | | Cecilie Uttrup Ludwig | CANYON//SRAM zondacrypto | +1:34m |
| 79 | | Mijntje Geurts | Team Visma | Lease a Bike | +1:34m |
| 80 | | Becky Storrie | Team Picnic PostNL | +1:35m |
| 81 |  | Petra Zsanko | Ceratizit-WNT Pro Cycling | +1:36m |
| 82 | | Brodie Chapman | UAE Team ADQ | +1:36m |
| 83 | | Eleonora Camilla Gasparrini | UAE Team ADQ | +1:39m |
| 84 |  | Mia Griffin | Roland | +1:40m |
| 85 | | Steffi Häberlin | Team SD Worx - Protime | +1:42m |
| 86 | | Amber Pate | Liv AlUla Jayco | +1:42m |
| 87 | | Katia Ragusa | Human Powered Health | +1:43m |
| 88 | | Erica Magnaldi | UAE Team ADQ | +1:44m |
| 89 | | Federica Damiana Piergiovanni | Isolmant - Premac - Vittoria | +1:44m |
| 90 | | Teuntje Beekhuis | Uno-X Mobility | +1:44m |
| 91 | | Marthe Truyen | Fenix-Deceuninck | +1:45m |
| 92 | | Babette van der Wolf | EF Education-Oatly | +1:45m |
| 93 | | Giada Borghesi | Human Powered Health | +1:46m |
| 94 | | Giorgia Vettorello | Roland | +1:46m |
| 95 | | Veronica Ewers | EF Education-Oatly | +1:48m |
| 96 | | Arianna Fidanza | Laboral Kutxa - Fundación Euskadi | +1:50m |
| 97 | | Carlotta Cipressi | Human Powered Health | +1:51m |
| 98 |  | Paula Patiño | Movistar Team | +1:52m |
| 99 | | Greta Marturano | UAE Team ADQ | +1:52m |
| 100 | | Célia Gery | FDJ - SUEZ | +1:52m |
| 101 | | Alice Maria Arzuffi | Laboral Kutxa - Fundación Euskadi | +1:55m |
| 102 | | Usoa Ostolaza | Laboral Kutxa - Fundación Euskadi | +1:57m |
| 103 | | Barbara Malcotti | Human Powered Health | +1:59m |
| 104 | | Rosita Reijnhout | Team Visma | Lease a Bike | +1:59m |
| 105 | | Iurani Blanco Calbet | Human Powered Health | +2:00m |
| 106 | | Laura Tomasi | Laboral Kutxa - Fundación Euskadi | +2:01m |
| 107 |  | Nina Berton | EF Education-Oatly | +2:02m |
| 108 | | Nora Jencusova | BePink - Imatra - Bongioanni | +2:07m |
| 109 | | Minke Anderson | Uno-X Mobility | +2:07m |
| 110 | | Giulia Giuliani | Roland | +2:08m |
| 111 | | Mirre Knaven | EF Education-Oatly | +2:08m |
| 112 | | Barbara Guarischi | Team SD Worx - Protime | +2:09m |
| 113 | | Ane Santesteban | Laboral Kutxa - Fundación Euskadi | +2:12m |
| 114 |  | Elisabeth Ebras | BePink - Imatra - Bongioanni | +2:15m |
| 115 | | Tiffany Cromwell | CANYON//SRAM zondacrypto | +2:15m |
| 116 | | Maya Kingman | EF Education-Oatly | +2:15m |
| 117 | | Meike Uiterwijk Winkel | BePink - Imatra - Bongioanni | +2:16m |
| 118 | | Linda Laporta | BePink - Imatra - Bongioanni | +2:17m |
| 119 | | Naia Amondarain | Laboral Kutxa - Fundación Euskadi | +2:21m |
| 120 | | Sara Pepoli | Isolmant - Premac - Vittoria | +2:24m |
| 121 | | Petra Stiasny | Roland | +2:25m |
| 122 | | Elisa Valtulini | BePink - Imatra - Bongioanni | +2:26m |
| 123 | | Chiara Reghini | Top Girls Fassa Bortolo | +2:27m |
| 124 | | Sara Luccon | Top Girls Fassa Bortolo | +2:32m |
| 125 | | Marta Pavesi | Top Girls Fassa Bortolo | +2:35m |
| 126 |  | Caroline Andersson | Liv AlUla Jayco | +2:36m |
| 127 | | Asia Zontone | Isolmant - Premac - Vittoria | +2:39m |
| 128 | | Gaia Segato | BePink - Imatra - Bongioanni | +2:39m |
| 129 |  | Argiro Milaki | Aromitalia 3T Vaiano | +2:40m |
| 130 | | Valeria Curnis | Isolmant - Premac - Vittoria | +2:44m |
| 131 | | Michela De Grandis | Team Mendelspeck E-Work | +2:44m |
| 132 | | Katelyn Nicholson | Team Mendelspeck E-Work | +2:45m |
| 133 | | Beatrice Rossato | Isolmant - Premac - Vittoria | +2:49m |
| 134 | | Giulia Vallotto | Team Mendelspeck E-Work | +2:53m |
| 135 | | Monica Castagna | Top Girls Fassa Bortolo | +2:58m |
| 136 |  | Rasa Leleivyte | Aromitalia 3T Vaiano | +3:01m |
| 137 | | Sofia Arici | Isolmant - Premac - Vittoria | +3:01m |
| 138 | | Andrea Casagranda | BePink - Imatra - Bongioanni | +3:04m |
| 139 | | Virginia Bortoli | Top Girls Fassa Bortolo | +3:06m |
| 140 | | Alice Bulegato | Top Girls Fassa Bortolo | +3:07m |
| 141 | | Vittoria Ruffilli | Roland | +3:08m |
| 142 | | Alessia Zambelli | Top Girls Fassa Bortolo | +3:15m |
| 143 | | Prisca Savi | Team Mendelspeck E-Work | +3:15m |
| 144 | | Emma Bernardi | Team Mendelspeck E-Work | +3:20m |
| 145 | | Hanna Tserakh | Aromitalia 3T Vaiano | +3:32m |
| 146 | | Irene Affolati | Aromitalia 3T Vaiano | +3:34m |
| 147 | | Sara Fiorin | Ceratizit-WNT Pro Cycling | +3:35m |
| 148 | | Maryna Altukhova | Team Mendelspeck E-Work | +3:40m |
| 149 | | Lucia Brillante Romeo | Aromitalia 3T Vaiano | +3:41m |
| 150 | | Emma Redaelli | Isolmant - Premac - Vittoria | +3:44m |
| 151 | | Chantelle McCarthy | Team Mendelspeck E-Work | +3:46m |
| 152 | | Fanny Bonini | Aromitalia 3T Vaiano | +3:51m |
| 153 |  | Valentina Venerucci | Aromitalia 3T Vaiano | +4:01m |
