| 1 | Marlen Reusser | Team SD Worx - Protime | 29:15m |
| 2 | Demi Vollering | Team SD Worx - Protime | +10s |
| 3 | Lotte Kopecky | Team SD Worx - Protime | +38s |
| 4 | Grace Brown | FDJ - SUEZ | +40s |
| 5 | Riejanne Markus | Team Visma | Lease a Bike | +50s |
| 6 | Juliette Labous | Team dsm-firmenich PostNL | +1:17m |
| 7 | Olivia Baril | UAE Team ADQ | +1:18m |
| 8 | Vittoria Guazzini | FDJ - SUEZ | +1:21m |
| 9 | Katarzyna Niewiadoma | Canyon//SRAM Racing | +1:23m |
| 10 | Lucinda Brand | Lidl - Trek | +1:30m |
| 11 | Audrey Cordon Ragot | Human Powered Health | +1:33m |
| 12 | Amber Kraak | Team Visma | Lease a Bike | +1:34m |
| 13 | Anna Henderson | Team Visma | Lease a Bike | +1:36m |
| 14 | Annemiek van Vleuten | Movistar Team | +1:41m |
| 15 | Georgina Howe | Liv AlUla Jayco | +1:41m |
| 16 | Coralie Demay | St Michel - Auber93 WE | +1:50m |
| 17 | Christina Schweinberger | Fenix-Deceuninck | +1:51m |
| 18 | Esmée Peperkamp | Team dsm-firmenich PostNL | +1:53m |
| 19 | Thalita de Jong | Liv Racing TeqFind | +1:56m |
| 20 | Mischa Bredewold | Team SD Worx - Protime | +2:03m |
| 21 | Anouska Koster | Uno-X Pro Cycling Team | +2:04m |
| 22 | Amanda Spratt | Lidl - Trek | +2:06m |
| 23 | Elizabeth Holden | UAE Team ADQ | +2:07m |
| 24 | Maaike Boogaard | AG Insurance - Soudal Team | +2:13m |
| 25 | Clara Koppenburg | Cofidis Women Team | +2:14m |
| 26 | Agnieszka Skalniak-Sójka | Canyon//SRAM Racing | +2:15m |
| 27 | Ricarda Bauernfeind | Canyon//SRAM Racing | +2:24m |
| 28 | Julie de Wilde | Fenix-Deceuninck | +2:26m |
| 29 | Karlijn Swinkels | Team Visma | Lease a Bike | +2:27m |
| 30 | Célia Le Mouel | St Michel - Auber93 WE | +2:27m |
| 31 | Josie Talbot | Cofidis Women Team | +2:27m |
| 32 | Georgia Williams | EF Education-Cannondale | +2:31m |
| 33 | Cedrine Kerbaol | Ceratizit-WNT Pro Cycling | +2:31m |
| 34 | Justine Ghekiere | AG Insurance - Soudal Team | +2:36m |
| 35 | Quinty Ton | Liv Racing TeqFind | +2:36m |
| 36 | Cecilie Uttrup Ludwig | FDJ - SUEZ | +2:37m |
| 37 | Pfeiffer Georgi | Team dsm-firmenich PostNL | +2:40m |
| 38 | Tamara Dronova-Balabolina | Israel Premier Tech Roland | +2:41m |
| 39 | Alena Amialiusik | UAE Team ADQ | +2:43m |
| 40 | Silvia Persico | UAE Team ADQ | +2:46m |
| 41 | Emma Norsgaard | Movistar Team | +2:46m |
| 42 | Sarah Roy | Canyon//SRAM Racing | +2:47m |
| 43 | Alexandra Manly | Liv AlUla Jayco | +2:48m |
| 44 | Eleonora Camilla Gasparrini | UAE Team ADQ | +2:48m |
| 45 | Ashleigh Moolman-Pasio | AG Insurance - Soudal Team | +2:52m |
| 46 | Liane Lippert | Movistar Team | +2:52m |
| 47 | Romy Kasper | AG Insurance - Soudal Team | +2:53m |
| 48 | Léa Curinier | Team dsm-firmenich PostNL | +2:56m |
| 49 | Silke Smulders | Liv Racing TeqFind | +2:57m |
| 50 | Marta Lach | Ceratizit-WNT Pro Cycling | +2:59m |
| 51 | Teniel Campbell | Liv AlUla Jayco | +3:01m |
| 52 | Floortje Mackaij | Movistar Team | +3:07m |
| 53 | Magdeleine Vallieres Mill | EF Education-Cannondale | +3:08m |
| 54 | Margot Pompanin | St Michel - Auber93 WE | +3:09m |
| 55 | Rachel Neylan | Cofidis Women Team | +3:10m |
| 56 | Evy Kuijpers | Fenix-Deceuninck | +3:14m |
| 57 | Simone Boilard | St Michel - Auber93 WE | +3:14m |
| 58 | Julia Borgström | AG Insurance - Soudal Team | +3:14m |
| 59 | Christine Majerus | Team SD Worx - Protime | +3:15m |
| 60 | Amber Pate | Liv AlUla Jayco | +3:18m |
| 61 | Sandra Alonso Dominguez | Ceratizit-WNT Pro Cycling | +3:19m |
| 62 | Ane Santesteban | Liv AlUla Jayco | +3:23m |
| 63 | Julie Van de Velde | Fenix-Deceuninck | +3:23m |
| 64 | Claire Steels | Israel Premier Tech Roland | +3:27m |
| 65 | Elizabeth Deignan | Lidl - Trek | +3:28m |
| 66 | Lauretta Hanson | Lidl - Trek | +3:29m |
| 67 | Megan Jastrab | Team dsm-firmenich PostNL | +3:30m |
| 68 | Dilyxine Miermont | St Michel - Auber93 WE | +3:32m |
| 69 | Hannah Ludwig | Uno-X Pro Cycling Team | +3:33m |
| 70 | Letizia Borghesi | EF Education-Cannondale | +3:35m |
| 71 | Jeanne Korevaar | Liv Racing TeqFind | +3:38m |
| 72 | Jade Wiel | FDJ - SUEZ | +3:38m |
| 73 | Ella Wyllie | Lifeplus–Wahoo | +3:38m |
| 74 | Alice Towers | Canyon//SRAM Racing | +3:40m |
| 75 | Elizabeth Stannard | Israel Premier Tech Roland | +3:40m |
| 76 | Sanne Cant | Fenix-Deceuninck | +3:42m |
| 77 | Maaike Coljé | Arkéa - B&B Hotels Women | +3:44m |
| 78 | Alison Jackson | EF Education-Cannondale | +3:44m |
| 79 | Elise Chabbey | Canyon//SRAM Racing | +3:46m |
| 80 | Elena Cecchini | Team SD Worx - Protime | +3:47m |
| 81 | Aude Biannic | Movistar Team | +3:47m |
| 82 | Kathrin Hammes | EF Education-Cannondale | +3:53m |
| 83 | Marthe Truyen | Fenix-Deceuninck | +3:54m |
| 84 | Sigrid Haugset | Team Coop - Repsol | +3:56m |
| 85 | Danielle De Francesco | Arkéa - B&B Hotels Women | +3:56m |
| 86 | Josie Nelson | Team Coop - Repsol | +4:02m |
| 87 | Alice Maria Arzuffi | Ceratizit-WNT Pro Cycling | +4:04m |
| 88 | Henrietta Christie | Human Powered Health | +4:05m |
| 89 | Clara Emond | Arkéa - B&B Hotels Women | +4:05m |
| 90 | Paula Patiño | Movistar Team | +4:09m |
| 91 | Arianna Fidanza | Ceratizit-WNT Pro Cycling | +4:10m |
| 92 | Marta Cavalli | FDJ - SUEZ | +4:11m |
| 93 | Sheyla Gutierrez | Movistar Team | +4:16m |
| 94 | Stine Dale | Team Coop - Repsol | +4:19m |
| 95 | Caroline Andersson | Liv Racing TeqFind | +4:19m |
| 96 | Barbara Malcotti | Human Powered Health | +4:23m |
| 97 | Nina Berton | Ceratizit-WNT Pro Cycling | +4:27m |
| 98 | Elena Hartmann | Israel Premier Tech Roland | +4:28m |
| 99 | Margaux Vigie | Lifeplus–Wahoo | +4:32m |
| 100 | Soraya Paladin | Canyon//SRAM Racing | +4:33m |
| 101 | Morgane Coston | Cofidis Women Team | +4:35m |
| 102 | Camille Fahy | St Michel - Auber93 WE | +4:37m |
| 103 | Erica Magnaldi | UAE Team ADQ | +4:39m |
| 104 | Kathrin Schweinberger | Ceratizit-WNT Pro Cycling | +4:39m |
| 105 | April Tacey | Lifeplus–Wahoo | +4:40m |
| 106 | Sandrine Bideau | St Michel - Auber93 WE | +4:40m |
| 107 | Wilma Olausson | Uno-X Pro Cycling Team | +4:41m |
| 108 | Typhaine Laurance | Lifeplus–Wahoo | +4:43m |
| 109 | India Grangier | Team Coop - Repsol | +4:43m |
| 110 | Tiril Jørgensen | Team Coop - Repsol | +4:58m |
| 111 | Anastasiya Kolesava | Arkéa - B&B Hotels Women | +5:05m |
| 112 | Yara Kastelijn | Fenix-Deceuninck | +5:14m |
| 113 | Sara Poidevin | EF Education-Cannondale | +5:17m |
| 114 | Natalie Grinczer | Lifeplus–Wahoo | +5:20m |
| 115 | Ilaria Sanguineti | Lidl - Trek | +5:21m |
| 116 | Coryn Labecki | Team Visma | Lease a Bike | +5:25m |
| 117 | Marjolein Van't Geloof | Human Powered Health | +5:28m |
| 118 | Susanne Andersen | Uno-X Pro Cycling Team | +5:50m |
| 119 | Jessica Allen | Liv AlUla Jayco | +5:54m |
| 120 | Nina Kessler | Liv AlUla Jayco | +5:55m |
| 121 | Antri Christoforou | Human Powered Health | +6:04m |
| 122 | Nathalie Eklund | Israel Premier Tech Roland | +6:13m |
| 123 | Eri Yonamine | Human Powered Health | +6:17m |
